Predicate: subclone
Roleset id: subclone.01 , reclone part of a previously-cloned DNA segment into a new vector, Source: , vncls: , framnet:
Aliases:
Alias | FrameNet | VerbNet |
subclone (v.) | | |
subclone (n.) | | |
Roles:
        Arg0-PAG: intentional agent of subcloning (e.g. scientist)
        Arg1-PPT: cloned entity
        Arg2-DIR: source, parent vector
        Arg3-GOL: destination vector
Example: intentional agent
        We subcloned two chicken H3 histone genes and transfected them into Rat 3 cell line.
        Arg0: We
        Rel: subcloned
        Arg1: two chicken H3 histone genes
Example: parent and destination vectors
        The gene encoding ɸ8 P4 helicase was subcloned from pET32 into pET28b for incorporation of an N-terminal His tag (pET28b-P4-His).
        Arg1: The gene encoding ɸ8 P4 helicase
        Rel: was subcloned
        Arg2: from pET32
        Arg3: into pET28b