Predicate: immunoblot

Roleset id: immunoblot.01 , analyse or identify proteins via antigen-antibody specific reactions, Source: , vncls: , framnet:

Aliases:

AliasFrameNetVerbNet
immunoblot (v.)
immunoblot (n.)

Roles:

        For Bio-AMR annotations.
        Arg0-PAG: intentional agent of immunoblotting (e.g. scientist)
        Arg1-PPT: target of immunoblotting, antigens/proteins checked for
        Arg2-PPT: source tissue being immunoblotted
        Arg3-MNR: instrument, antibody, stain

Example: Arg0

        In our characterization of the DOR antisera, we immunoblotted protein extracted from NG108-15 cells and rat spinal cord.

        Arg0: we
        Arg1: protein extracted from NG108-15 cells and rat spinal cord

Example: Arg3

        Bound proteins were resolved by SDS-PAGE and immunoblotted with anti-BCL6 and anti-c-Rel antibodies.

        Arg1: Bound proteins
        Arg3: anti-BCL6 and anti-c-Rel antibodies

Example: Arg2

        TRUSS was immunoprecipitated and immunoblotted from HeLa cells that stably expressed Flag-TRUSS.

        Arg1: TRUSS
        Arg2: HeLa cells that stably expressed Flag-TRUSS