Predicate: dephosphorylate

Roleset id: dephosphorylate.01 , remove a phosphate group from an organic compound by hydrolysis, Source: , vncls: , framnet:

dephosphorylate.01: DEPHOSPHORYLATE-V NOTES: Created for Bio-AMR annotations.

Aliases:

AliasFrameNetVerbNet
dephosphorylate (v.)
dephosphorylation (n.)

Roles:

        Arg0-PAG: intentional agent of dephosphorylation (e.g. scientist)
        Arg1-PPT: thing dephosphorylated
        Arg2-MNR: instrument
        Arg3-PRD: product of dephosphorylation

Example: dephosphorylate-v: passivized, with product of the reaction

        Regarding the relationship between adenosine and ATP, []intracellular adenosine]-1 is dephosphorylated *-1 from AMP by cytosolic 5'-nucleotidase and is converted back to AMP via adenosine kinase (ADK).

        Argm-adv: Regarding the relationship between adenosine and ATP
        Rel: dephosphorylated
        Arg3: *-1
        Arg1: from AMP
        Arg2: by cytosolic 5'-nucleotidase

Example: dephosphorylate-v: agent and instrument

        For cloning, we dephosphorylate the RNAs with shrimp nuclease and deproteinate the reaction with proteinase K, followed by phenol/chloroform extraction.

        Argm-prp: for cloning
        Arg0: we
        Rel: dephosphorylated
        Arg1: the RNAs
        Arg2: with shrimp nuclease

Example: dephosphorylation-n

        Dephosphorylation of the 5' end prohibits self-ligation, enabling the researcher to manipulate the DNA as desired before re-ligating.

        Rel: Dephosphorylation
        Arg1: of the 5' end