Predicate: denature

Roleset id: denature.01 , modify the molecular structure (of a protein or other biological macromolecule), Source: , vncls: , framnet:


denature (v.)
denaturation (n.)


        For Bio-AMR annotations.
        Arg0-PAG: intentional agent of denaturation (e.g. scientist)
        Arg1-PPT: thing denatured
        Arg2-GOL: end state
        Arg3-DIR: start state
        Arg4-MNR: instrument, denaturant

Example: example with agent

        We denatured the RNA/primer combinations, assembled the reactions and incubated for coupled RT-PCR.

        Arg0: we
        Arg1: the RNA/primer combinations

Example: example with instrument

        In Anfinsen's experiment, RNAse was denatured with urea and β-mercaptoethanol.

        Arg1: RNAse
        Arg4: urea and β-mercaptoethanol

Example: example with start state and end state

        The target DNA is denatured from dsDNA to ssDNA by heating to about 95°C.

        Arg1: The target DNA
        Arg2: ssDNA
        Arg3: dsDNA