Predicate: biotinylate

Roleset id: biotinylate.01 , attach a biotin residue to a protein or other molecule, Source: , vncls: , framnet:

Aliases:

AliasFrameNetVerbNet
biotinylate (v.)
biotinylation (n.)

Roles:

        Arg0-PAG: intentional agent of biotinylation (e.g. scientist)
        Arg1-PPT: thing biotinylated (e.g. protein)
        Arg2-MNR: instrument
        Arg3-PRD: result of biotinylation

Example: intentional agent

        To facilitate the discrimination between human serum proteins and immunoprecipitated antigens we biotinylated proteins of liver cytosol.

        Arg0: we
        Rel: biotinylated
        Arg1: proteins of liver cytosol

Example: example with instrument

        The purified oligonucleosomes are then biotinylated with an amine-reactive reagent, before dialysis into the final storage buffer.

        Arg1: The purified oligonucleosomes
        Rel: are biotinylated
        Arg2: with an amine-reactive reagent

Example: product of reaction

        Thus, the 264scTCR/birA fusion protein can be biotinylated to create 264scTCR/birA monomers, which can subsequently be used to form 264scTCR/multimers in the presence of streptavidin.

        Arg1: the 264scTCR/birA fusion protein
        Rel: be biotinylated
        Arg3: 264scTCR/birA monomers